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KMID : 1134820080370080992
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Journal of the Korean Society of Food Science and Nutrition
2008 Volume.37 No. 8 p.992 ~ p.997
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Inhibitory Effect of Conditioned Medium of Silk Fibroin-Treated Osteoblasts in Osteoclast Differentiation
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Yeo Joo-Hong
Kim Sung-Su
Park Kyung-Ho
Lee Kwang-Gill
Ju Won-Chul
Lee Jin-Ah
Woo Soon-Ok
Han Sang-Mi
Kweon Hae-Yong
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Abstract
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In this study, we investigated the indirect effect of silk-fibroin on osteoclastic differentiation of RAW264.7
cells. The conditioned medium were collected from MC3T3-E1 osbeoblasts treated with 0.001 mg/mL～0.1
mg/mL silk fibroin for 6 days, mixed in 1:1 ratio with osteoclast medium, and then added into RAW264.7 cells
with receptor activator of nuclear factor kappa B ligand (RANKL), a differentiation inducer for 3 days. Of
osteoclastic cytokines in the conditioned medium, the protein expression of osteoprotegerin (OPG) with silk-fibroin
was not significantly different. However, the protein expression of interleukin (IL)-1β was specifically
lower in a dose dependent manner. In RAW264.7 cells, the conditioned medium with silk-fibroin inhibited
RANKL induced osteoclastic differentiation as total number of multinucleated tartrate-resistant alkaline phosphatase
(TRAP)-positive osteoclasts in a dose dependent manner. Taken together, we demonstrated that the
conditioned medium of silk-fibroin treated osteoblasts inhibits RANKL induced differentiation of osteoclasts
with inhibiting selective expression of IL-1β.
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KEYWORD
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silk-fibroin, conditioned medium, osteoprotegerin, interleukin-1β, osteoclastic differentiation
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